Módulo 6- Mass spectrometry based proteomics Advanced Computational Proteomics and Metabolomics

Módulo 6- Mass spectrometry based proteomics Advanced Computational Proteomics and Metabolomics
5,00 €

Summary with answered questions

Index
  • Proteomics vs genomics 
  • Abundance 
    • Static blueprint. 
    • What could happen. 
    • Indicates cellular intent.
    • What might happen 
    • Functional reality. 
    • What is actually happening 
    • Multilayered Proteomics 
    • Clinical proteomics 
    • The use of mass spectrometry (MS) -based proteomics 
    • Questions 
  • LC-MS pipeline (hight level)- Liquid Chromatography-Mass
    • Spectrometry 
    • The LC-ESI-MS/ MS Bottom-up proteomics 
    • Biological Sample Inputs 
    • Sample Fractionation Strategies 
    • Alkylation and Trypsin Digestion 
    • The Tandem Mass Spectrometer (MS/MS) 
      • 1. Electro spray Ionization (ESI) 
      • 2. The MS1 Analyzer: The Quadrupole 
      • 4. The Collision Cell (CID/ HCD) 
      • 5. The MS2 Analyzer: The Orbitrap 10
      • 5. The MS2 Analyzer: Time-of-Flight (TOF) 
      • The Duty Cycle: MS1 to MS2
      • Questions
  • The spectra 
    • Peptide Fragmentation: b and y Ions 
    • Active Check point: Reading the Spectrum
    • Peptide de novo sequencing 
  • Summary: The Bottom-Up Proteomics Pipeline 
  • Questions 
  • The algorithm 
    • Data base dependent search
    • Search strategy
    • Scoring of peptide spectrum matches
    • False discovery rate estimation (FDR)
    • Protein inference
    • Summary: Peptide & Protein ID
    • Questions

Your feedback is important!

Classificação: 0 estrelas
0 votos

Adicionar comentário

Comentários

Ainda não há comentários.