Módulo 1: High-throughput Sequencing Technologies I

High-throughput Sequencing Technologies (HTS): 

• How to measure gene expression (mRNA levels)? 
• Illumina Sequencing 

Different HTS Technologies

• Long-reads sequencing
  • Nanopore 

HTS Applications

• GENOME STATE / DNA EXPRESSION 
  • Whole-Genome sequencing (WGS)
  • Whole-Exome sequencing (WES)
  • ChIP-seq- Protein-DNA interacions 
  • Single-Cell Sequencing 
  • Chromosome Conformation Capture (3C) 
  • Others applications 
• RNA LIFE CYCLE – RNA EXPRESSION 
  • RNA-seq- Transcriptome
  • miRNA-seq 
  • Specialized HTS variants applied to RNA 
  • Spatial Transcriptomics (ST) 
  • Others applications 

HTS Data Analysis 

• Multi-Omics Resources 
  • NCBI- using SRA (Sequence Read Archive
• Layout: Single-end and Paired-end Read 
• Raw data file 
• Quality score 
• Alignment/ Assembly 
• View Alignment 
• Genome Browsers